کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5286190 1385775 2007 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Easy separation of optically active products by enzymatic hydrolysis of soluble polymer-supported substrates
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Easy separation of optically active products by enzymatic hydrolysis of soluble polymer-supported substrates
چکیده انگلیسی
The easy separation of optically active compounds from enzymatic kinetic resolution products by simple precipitation using poly(ethylene glycol)(PEG)-supported carbonates is disclosed. The water-soluble substrate was prepared by the immobilization of (±)-1-phenylethanol onto a middle-molecular weight (av Mw 5000) monomethoxy PEG (MPEG) through a carbonate linker. The enantioselective hydrolysis using Lipase from porcine pancreas (PPL; Type II, Sigma) in a mixed solvent (hexane/buffer = 9:1) proceeded to afford the corresponding optically active compounds. In this system, the separation of the products was achieved by a simple procedure without laborious column chromatography. A hydrophobic spacer between the MPEG moiety and the carbonate linker affected both the reactivity and enantioselectivity, and the substrate with a phenylethyl spacer was hydrolyzed with the highest enantioselectivity (E value = 270).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tetrahedron Letters - Volume 48, Issue 48, 26 November 2007, Pages 8540-8543
نویسندگان
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