In vitro study of cell differentiation by two type mouse embryo stem cells on mono- and multilayer nanocarbon tubes

► We previously investigated the myocardial cell differentiation of ES-D3 cells using monolayer (SWCNTs) and multilayer (MWCNTs) nanocarbon tubes. As a result, in spite of having the same carbon composition, the effects on the cell differentiation levels differed between the tubes. ► We investigated their cell differentiation and cytotoxic effects on EL M3 and ES-R1-EGFP B2/EGFP cells, which require feeder cells. ► The different surface structures of SWCNTs and MWCNTs may have influenced ES cell differentiation.