Immobilization of naringinase on mesoporous molecular sieve MCM-41 and its application to debittering of white grapefruit

▶ In the present report, naringinase has been adsorbed on to MCM-41 by cross linking agent glutaraldehyde. ▶ The results showed that the immobilized preparation of naringinase seemed to be very suitable for debitterring of grapefruit. ▶ The properties of the free and immobilized naringinase such as hydrolytic activity, thermal stability, reusability and kinetic behavior were investigated, using naringin hydrolysis. ▶ The impact of naringin enzymatic hydrolysis in the white grapefruits was evaluated. ▶ Furthermore, the molecular sieve MCM-41 and MCM41-naringinase matrix were characterized by FT-IR, elemental analysis and the nitrogen adsorption-desorption isotherms.