کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5370597 1503892 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rigorous analysis of static light scattering measurements on buffered protein solutions
ترجمه فارسی عنوان
تجزیه و تحلیل دقیق از اندازه گیری پراکندگی نور استاتیک در محلول پروتئین بافر
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
چکیده انگلیسی


- Expressions for rigorous thermodynamic analysis of static light scattering measurements on buffered protein solutions
- Buffer components and supporting electrolytes must be regarded as additional cosolutes rather than part of the solvent
- Effects of small cosolute inclusion on molecular mass estimation are evident in published light scattering data
- Allowance for nonideality by currently used single-solute theory is of questionable value

Attention is drawn to the thermodynamic invalidity of the current practice of analyzing static light scattering measurements on globular proteins in terms of theory for a single solute because of its disregard of the need to consider small species such as buffer components as additional cosolutes rather than as part of the solvent. This practice continues despite its demonstrated inadequacy in studies of sucrose-supplemented protein solutions, where the aberrant behavior was recognized to be a consequence of physical protein interaction with the small cosolute. Failure to take into account the consequences of small cosolute effects renders extremely difficult any attempt to obtain a rigorous thermodynamic characterization of protein interactions by this empirical technique.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 228, September 2017, Pages 108-113
نویسندگان
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