کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5370603 1503891 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Spectroscopic characterization and fluorescence imaging of Helicobacter pylori endogenous porphyrins
ترجمه فارسی عنوان
خصوصیات اسپکتروسکوپی و تصویربرداری فلورسانس از پورفیرین های اندوژن هلیکوباکتر پیلوری
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
چکیده انگلیسی


- Helicobacter pylori (Hp) produces porphyrins acting as endogenous photosensitizers.
- PhotoDynamic Therapy can be a promising treatment for Hp eradication.
- Hp endogenous porphyrins have been spectroscopically characterized.
- The fluorescence properties of Hp biofilm have been studied for the first time.

Conventional antimicrobial strategies have become increasingly ineffective due to the rapid emergence of antibiotic resistance among pathogenic bacteria. In order to overcome this problem, antimicrobial PhotoDynamic Therapy (PDT) is considered a promising alternative therapy. PDT has a broad spectrum of action and low mutagenic potential. It is particularly effective when microorganisms present endogenous photosensitizing pigments. Helicobacter pylori (Hp), a pathogen notoriously responsible of severe gastric infections (chronic gastritis, peptic ulcer, MALT lymphoma and gastric adenocarcinoma), produces and accumulates the photosensitizers protoporphyrin IX and coproporphyrin, thus it might be a suitable target of antimicrobial PDT. With the aim to design and develop an ingestible LED-based robotic pill for intragastric phototherapy, so that irradiation can be performed in situ without the use of invasive endoscopic light, photophysical studies on the Hp endogenous photosensitizers were carried out. These studies represent an important prerequisite in order to select the most effective irradiation conditions for Hp eradication. The photophysical characterization of Hp porphyrins, including their spectroscopic features in terms of absorption, steady-state and time-resolved fluorescence, was performed on bacterial extracts as well as within planktonic and biofilm growing Hp cells.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 229, October 2017, Pages 19-24
نویسندگان
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