کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5370648 1503898 2017 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Signal and binding. II. Converting physico-chemical responses to macromolecule-ligand interactions into thermodynamic binding isotherms
ترجمه فارسی عنوان
سیگنال و اتصال دوم تبدیل واکنش های فیزیکی شیمیایی به تعاملات لیزر ماکرومولکول-لیگاند به ایزوترم های اتصال ترمودینامیکی
کلمات کلیدی
تابعهای فیزیکی شیمیایی، ایزوترمهای مرتبط ترمودینامیک،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
چکیده انگلیسی


- In general, there is no linear relationship between observed physico-chemical signals and the total average degree of binding.
- Signal and mass conservation relationships allow the construction of binding isotherms from physico-chemical titration curves.
- Ligand Binding Density Function (LBDF) Method allows the construction of binding isotherms using the ligand signal.
- The Empirical Function (EF) Method relates the observed signal to the total average degree of binding.

Physico-chemical titration techniques are the most commonly used methods in characterizing molecular interactions. These methods are mainly based on spectroscopic, calorimetric, hydrodynamic, etc., measurements. However, truly quantitative physico-chemical methods are absolutely based on the determination of the relationship between the measured signal and the total average degree of binding in order to obtain meaningful interaction parameters. The relationship between the observed physico-chemical signal of whatever nature and the degree of binding must be determined and not assumed, based on some ad hoc intuitive relationship/model, leading to determination of the true binding isotherm. The quantitative methods reviewed and discussed here allow an experimenter to rigorously determine the degree of binding and the free ligand concentration, i.e., they lead to the construction of the thermodynamic binding isotherm in a model-independent fashion from physico-chemical titration curves.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 222, March 2017, Pages 25-40
نویسندگان
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