کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5370912 | 1503918 | 2015 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A look at the effect of sequence complexity on pressure destabilisation of DNA polymers A look at the effect of sequence complexity on pressure destabilisation of DNA polymers](/preview/png/5370912.png)
- Under certain conditions synthetic nucleic polymers can be denatured with pressure.
- We show that the change of TM of Clostridium perfringes DNA with pressure is negative.
- We were unable to induce a helix-coil transition C. perfringes DNA with pressure.
- The sequence complexity of this genomic DNA underlies its insensitivity to pressure.
Our previous studies on the helix-coil transition of double-stranded DNA polymers have demonstrated that molar volume change (ÎV) accompanying the thermally-induced transition can be positive or negative depending on the experimental conditions, that the pressure-induced transition is more cooperative than the heat-induced transition [Rayan and Macgregor, J Phys Chem B 2005, 109, 15558-15565], and that the pressure-induced transition does not occur in the absence of water [Rayan and Macgregor, Biophys Chem, 2009, 144, 62-66]. Additionally, we have shown that ÎV values obtained by pressure-dependent techniques differ from those obtained by ambient pressure techniques such as PPC [Rayan et al. J Phys Chem B 2009, 113, 1738-1742] thus shedding light on the effects of pressure on DNA polymers. Herein, we examine the effect of sequence complexity, and hence cooperativity on pressure destabilisation of DNA polymers. Working with Clostridium perfringes DNA under conditions such that the estimated ÎV of the helix-coil transition corresponds to â 1.78 mL/mol (base pair) at atmospheric pressure, we do not observe the pressure-induced helix-coil transition of this DNA polymer, whereas synthetic copolymers poly[d(A-T)] and poly[d(I-C)] undergo cooperative pressure-induced transitions at similar ÎV values. We hypothesise that the reason for the lack of pressure-induced helix-coil transition of C. perfringens DNA under these experimental conditions lies in its sequence complexity.
Journal: Biophysical Chemistry - Volume 199, April 2015, Pages 34-38