کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5371177 | 1503940 | 2012 | 8 صفحه PDF | دانلود رایگان |
In cells, an appreciable part of enzymes is associated with lipid membranes. Academic experimental studies of the function of membrane enzymes (e.g., PLA2 representing a prototype for interfacial enzymology) are often focused on the enzyme-vesicle interaction or, more specifically, on conversion of lipid forming the external leaflet of the vesicle membrane. The corresponding kinetics are complicated by many factors inherent to the interfacial physics and chemistry. The understanding of the relative role of such factors and how they should be quantitatively described is still limited. Here, we present the mean-field kinetic equations, taking the formation of rafts in the membrane and the product-induced membrane strain into account, and analyze various scenarios of lipid conversion. In particular, we scrutinize the conditions when the kinetics may exhibit a transition from a relatively long latency period to a steady-state regime with fast nearly constant reaction rate. Specifically, we discuss the likely role of the pore formation in the external lipid layer in this transition. The latter effect may be caused by the product-induced tensile strain in this layer.
Highlights⺠The analysis is focused on enzyme-catalyzed hydrolysis of lipids. ⺠The formation of rafts composed of product is taken into account. ⺠The product-induced membrane strain is taken into account as well. ⺠Various scenarios of lipid conversion are described. ⺠The conditions of the appearance of the lag phase are scrutinized.
Journal: Biophysical Chemistry - Volume 170, AugustâSeptember 2012, Pages 17-24