کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5371239 | 1503943 | 2012 | 8 صفحه PDF | دانلود رایگان |

It was first found that alkali metal poly(l-glutamate)s show the coil-globule transition and the coil-helix transition sequentially in aqueous ethanol with increasing the solvent concentration. The counterion specificity for the former transition, i.e., Na+Â >Â K+, Rb+Â >Â Li+, Cs+, proved to be somewhat different from that for the latter; Na+Â >Â Li+Â >Â K+Â >Â Rb+Â >Â Cs+. Counterion mixing effects were also observed for both transitions; the most effective combinations to induce the transitions were Li+/Cs+ and Li+/K+, respectively. Solution viscometry, circular dichroism and alkali metal NMR line width measurements for the single-counterion systems revealed that the contact ion-pair formation hardly occurs for Li+ even at the collapsed globule state and in the helix conformation. Unexpectedly, however, the specific binding of Li+ was induced just by mixing with K+ or Cs+, when the helix content concomitantly increased. Mechanism for the counterion-specific “double transition” as well as the counterion mixing effect is discussed referring to the size-fitting model that has been proposed for the coil-globule transition of alkali metal poly(acrylate)s.
Highlights⺠Alkali metal poly(l-glutamate) shows “globule-helix” transition in aqueous ethanol. ⺠Mixing of smaller and larger counterions induces α-helix formation. ⺠Contact-ion pair formation is not necessary for α-helix formation.
Journal: Biophysical Chemistry - Volumes 165â166, May 2012, Pages 48-55