کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5371424 | 1388819 | 2010 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Self-organization of G-quadruplex structures in the hTERT core promoter stabilized by polyaminic side chain perylene derivatives Self-organization of G-quadruplex structures in the hTERT core promoter stabilized by polyaminic side chain perylene derivatives](/preview/png/5371424.png)
hTERT core promoter regulates telomerase transcription in human cells, thus its structural features are of large interest. We have found that the G-rich hTERT core promoter region, corresponding to the major DNase I hypersensitive site in chromatin organization, contains nine putative G-quadruplex forming sequences (PQS) and is unfavorable for nucleosome formation. Here we show that four PQS are effectively able to form stable parallel intramolecular G-quadruplexes, using PAGE and CD spectroscopy analysis. The PQS-region, as a whole, appears to be organized in three self-interacting G-quadruplexes, probably giving rise to a helicoidal superstructure, as shown by CD and polymerase stop assay.POL-HPDI drugs, that we previously found useful in selectively stabilizing telomeric G-quadruplex, are able to stabilize both the single intramolecular G-quadruplex and the PQS-region superstructure. The features of their induced CD spectra suggest that POL-HPDIs bind to single G-quadruplexes and to whole PQS-region superstructure, mainly by end-stacking interactions.
Graphical AbstractResearch Highlights⺠The hTERT core promoter contains nine putative G-quadruplex forming sequences (PQS). ⺠Four PQS are effectively able to form parallel intramolecular G-quadruplexes. ⺠The PQS-region appears to be organized in three self-interacting G-quadruplexes. ⺠POL-HPDIs stabilize both the single G-quadruplexes and the PQS-region superstructure.
Journal: Biophysical Chemistry - Volume 153, Issue 1, December 2010, Pages 43-53