Two-state displacement by the kinesin-14 Ncd stalk

►Rotation of the kinesin-14 Ncd stalk represents the motor power stroke. ►Structural data imply that stalk movement is required for motor-microtubule binding. ►We show that the stalk rotates close to the microtubule upon motor binding to ATP. ►Ncd stalk movement or rotation is thus proposed to occur in two steps. ►This would produce a working stroke and step larger than the kinesin-1 8-nm step.