کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5371694 1503964 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dynamic force spectroscopy of the Helicobacter pylori BabA-Lewis b Binding
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Dynamic force spectroscopy of the Helicobacter pylori BabA-Lewis b Binding
چکیده انگلیسی
The binding strength of the Helicobacter pylori adhesin-receptor complex BabA-ABO/Lewis b has been analyzed by means of dynamic force spectroscopy. High-resolution measurements of rupture forces were performed in situ on single bacterial cells, expressing the high-affinity binding BabA adhesin, by the use of force measuring optical tweezers. The resulting force spectra revealed the mechanical properties of a single BabA-Leb bond. It was found that the bond is dominated by one single energy barrier and that it is a slip-bond. The bond length and thermal off-rate were assessed to be 0.86 ± 0.07 nm and 0.015 ± 0.006 s− 1, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 143, Issues 1–2, July 2009, Pages 102-105
نویسندگان
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