کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5371804 | 1388844 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of quinone on the fluorescence decay dynamics of endogenous flavin bound to bacterial luciferase
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effect of quinone on the fluorescence decay dynamics of endogenous flavin bound to bacterial luciferase Effect of quinone on the fluorescence decay dynamics of endogenous flavin bound to bacterial luciferase](/preview/png/5371804.png)
چکیده انگلیسی
The interaction of quinone with luciferase from Photobacterium leiognathi was studied based on the fluorescence decay measurements of the endogenous flavin bound to the enzyme. Homologous 1,4-quinones, 1,4-benzoquinone, methyl-1,4-benzoquinone, 2-methyl-5-isopropyl-1,4-benzoquine and 1,4-naphthoquinone, were investigated. In the absence of quinone, the fluorescence intensity and anisotropy decays of the endogenous flavin exhibited two intensity decay lifetimes (~Â 1 and 5Â ns) and two anisotropy decay lifetimes (~Â 0.2 and 20Â ns), suggesting a heterogeneous quenching and a rotational mobility microenvironment of the active site of the luciferase, respectively. In the presence of quinone, the intensity decay heterogeneity was largely maintained, whereas the fraction of the short anisotropy decay component and the averaged rotational rate of FMN increased with the increasing hydrophobicity of the quinone. We hypothesize that the hydrophobicity of the quinone plays a role in the non-specific inhibition mechanism of xenobiotic molecules in the bacterial bioluminescence system via altering the rotational mobility of the endogenous flavin in the luciferase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 141, Issue 1, April 2009, Pages 59-65
Journal: Biophysical Chemistry - Volume 141, Issue 1, April 2009, Pages 59-65
نویسندگان
Elena V. Vetrova, Nadezhda S. Kudryasheva, Kwan H. Cheng,