کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5372043 1503972 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tricationic pyridium porphyrins appending different peripheral substituents: Experimental and DFT studies on their interactions with DNA
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Tricationic pyridium porphyrins appending different peripheral substituents: Experimental and DFT studies on their interactions with DNA
چکیده انگلیسی

Four tricationic pyridium porphyrins appending hydroxyphenyl, methoxyphenyl, propionoxyphenyl or carboxyphenyl group at meso-20-position of porphyrin core have been synthesized and their abilities to bind and cleave DNA have been investigated. Using a combination of absorption, fluorescence, circular dichroism (CD) spectra, thermal DNA denaturation as well as viscosity measurements, their binding modes and intrinsic binding constants (Kb) to calf DNA (CT DNA) were comparatively studied and also compared with those of 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin (TMPyP). The results suggest that the Kb values of these porphyrins are greatly influenced by the number of positive charges and steric hindrance. Theoretical calculations applying the density functional theory (DFT) have been carried out and explain their DNA-binding properties reasonably. The efficiency of DNA photocleavage by these porphyrins shows high dependence on the values of Kb.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 135, Issues 1–3, June 2008, Pages 102-109
نویسندگان
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