کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5372153 1503982 2007 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structure-specific recognition of quadruplex DNA by organic cations: Influence of shape, substituents and charge
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Structure-specific recognition of quadruplex DNA by organic cations: Influence of shape, substituents and charge
چکیده انگلیسی

Combining structure-specific recognition of nucleic acids with limited sequence reading is a promising method to reduce the size of the recognition unit required to achieve the necessary selectivity and binding affinity to control function. It has been demonstrated recently that G-quadruplex DNA structures can be targeted by organic cations in a structure-specific manner. Structural targets of quadruplexes include the planar end surfaces of the G-tetrad stacked columns and four grooves. These provide different geometries and functional groups relative to duplex DNA. We have used surface plasmon resonance and isothermal titration calorimetry to show that binding affinity and selectivity of a series of quadruplex end-stacking molecules to human telomeric DNA are sensitive to compound shape as well as substituent type and position. ITC results indicate that binding is largely enthalpy driven. Circular dichroism was also used to identify a group of structurally related compounds that selectively target quadruplex grooves.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 126, Issues 1–3, March 2007, Pages 140-153
نویسندگان
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