کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5372292 | 1503983 | 2007 | 9 صفحه PDF | دانلود رایگان |
Phycocyanin is a phycobiliprotein involved in light harvesting and conduction of light to the reaction centers in cyanobacteria and red algae. The structure of C-phycocyanin from Gracilaria chilensis was solved by X-ray crystallography at 2.0 à resolution in space group P21. An interaction model between two PC heterohexamers was built, followed by molecular dynamic refinement. The best model showed an inter-hexamer rotation of 23°. The coordinates of a PC heterohexamer (αβ)6 and of the PC-PC complex were used to perform energy transfer calculations between chromophores pairs using the fluorescence resonance energy transfer approach (FRET). Two main intra PC (Iβ382 â Iα184 â Iα584 â Iβ682 and Iβ3153 â Iβ5153) and two main inter PC (Iβ682 â IIβ382 and Iβ5153 â IIβ3153) pathways were proposed based on the values of the energy transfer constants calculated for all the chromophore pairs in the hexamer and in the complex.
Journal: Biophysical Chemistry - Volume 125, Issues 2â3, February 2007, Pages 388-396