کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5372322 1503980 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of insulin amyloid fibrils by Raman spectroscopy
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Analysis of insulin amyloid fibrils by Raman spectroscopy
چکیده انگلیسی

The formation of amyloid fibrils from insulin is investigated using drop-coating-deposition-Raman (DCDR) difference spectroscopy and atomic force microscopy (AFM). Fibrils formed using various co-solvents and heating cycles are found to induce the appearance of Raman difference peaks in the amide I (∼ 1675 cm− 1), amide III (∼ 1220 cm− 1), and peptide backbone (∼ 1010 cm− 1), consistent with an increase in β-sheet content. Comparisons of results obtained from fibrils in either H2O or D2O suggest that the NH/ND stretch bands (at ∼ 3300 cm− 1/∼ 2400 cm− 1) are also enhanced in intensity upon fibril formation. If there is any water trapped in the core of the fibrils its OH/OD Raman intensity is too small to be detected in the presence of the stronger NH/ND bands which appear in the same region. AFM is used to confirm the formation of fibrils of about 5 nm diameter (and various lengths).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 128, Issues 2–3, July 2007, Pages 150-155
نویسندگان
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