کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5372482 1388884 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Multithermal titration calorimetry: A rapid method to determine binding heat capacities
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Multithermal titration calorimetry: A rapid method to determine binding heat capacities
چکیده انگلیسی

Herein a new method that allows binding ΔCp to be determined with a single experiment is presented. Multithermal titration calorimetry (MTC) is a simple extension of isothermal titration calorimetry (ITC) that explicitly takes into account the thermal dependences of ΔH and the binding constant. Experimentally, this is accomplished by performing a single stepwise titration with ITC equipment, allowing temperature re-adjustments of the system at intermediate states of the titration process. Thus, from the resulting multitherm, ΔCp can also be determined. The experimental feasibility of MTC was tested by using the well-characterized lysozyme-chitotriose complex as a model system.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 120, Issue 1, 1 March 2006, Pages 10-14
نویسندگان
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