کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5372544 1388890 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Vibrational circular dichroism signature of hemiprotonated intercalated four-stranded i-DNA
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Vibrational circular dichroism signature of hemiprotonated intercalated four-stranded i-DNA
چکیده انگلیسی

The four-stranded intercalated DNA structure exemplified by the oligonucleotide 5′-d(CCCCCCCCCCCC) (d(C)12) was studied at acidic pH by infrared absorption (IR) and vibrational circular dichroism (VCD) spectroscopy and compared with spectra of the same oligonucleotide at neutral pH to establish distinct VCD markers for the intercalation motif. The most striking feature is a new absorption at 1694 cm− 1 and its corresponding VCD couplet with reversed sign. These are unique for the intercalated structure and have not been observed for other parallel stranded duplexes. Significant characteristic features resulting from the spatial arrangement of the sugar-phosphate backbone are also clearly present for d(C)12 at acidic pH. An extensive network of CH…O bonds twists the backbone such that multiple through-space vibrational coupling occurs among neighbouring sugar-phosphate residues resulting in unusual VCD signals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 119, Issue 1, 1 January 2006, Pages 1-6
نویسندگان
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