کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5373371 1504216 2015 36 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Water rotation barriers on protein molecular surfaces
ترجمه فارسی عنوان
موانع چرخش آب بر روی سطوح مولکولی پروتئین
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
چکیده انگلیسی
The experimental characterization of hindered-rotation barriers and mapping the energetic heterogeneity of water molecules bound to the molecular “surface” of proteins is critical for understanding the functional interaction of proteins with their environment. Here, we show how to achieve this goal by an original wide-line NMR procedure, which is based on the spectral motional narrowing phenomenon following the melting (thawing) process of interfacial ice. The procedure highlights the differences between globular and intrinsically disordered proteins and it enables to delineate the effect of solvent on protein structure, making a distinction between point mutants, monomeric and oligomeric states, and characterizing the molecular interactions taking part in different cellular processes. We put this unique experimental approach introducing novel physical quantities and quantifying the heterogeneous distribution of motional activation energy of water in the interfacial landscape into a historical perspective, demonstrating its utility through a variety of globular and disordered proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Physics - Volume 448, 20 February 2015, Pages 15-25
نویسندگان
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