Solution NMR spectroscopy of supra-molecular systems, why bother? A methyl-TROSY view

► Explanation of how dipolar cross-correlated relaxation in a 13CH3 group can be exploited for studies of supra-molecular complexes. ► Studies of the proteasome via methyl-TROSY NMR emphasize the importance of dynamics for the function. ► Studies of proteasome substrates show that they are maintained in a disordered state inside the antechamber.