کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5434715 1509142 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A simple semi-quantitative approach studying the in vivo degradation of regenerated silk fibroin scaffolds with different pore sizes
ترجمه فارسی عنوان
یک رویکرد ساده نیمه کمی که در حال بررسی تخریب درون فیبروئن ابریشم بازسازی شده با اندازه های مختلف منافذ
کلمات کلیدی
تخریب درونی، داربست فیبرین ابریشم، فیبروبلاست، التهاب اندازه منفذ،
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی


- The new semi-quantitative approach was demonstrated to be a simple and effective method.
- Biodegradation of regenerated silk fibroin scaffolds (RSFs) was inflammation-mediated to a certain degree.
- Fibroblasts may play a critical role in biodegradation of RSFs.
- RSFs with both pore sizes (74.35 ± 10.84 μm and 139.23 ± 44.93 μm, respectively) were well tolerated by host animals.
- Pore size was a rate limiting factor to the biodegradation in a subcutaneous implantation model.

The biocompatibility and in vivo degradation rate of biomaterials represent critical control points in the long-term success of scaffolds for tissue restoration. In this study, new three-dimensional (3D) regenerated silk fibroin scaffolds (RSFs) were prepared by the freezing-defrosting procedure, and then were implanted beneath the dorsal skin of rats. This study aims to develop a kinetic semi-quantitative approach to assess in vivo degradation rate and biocompatibility of this kind of RSFs with different pore sizes for the first time, and to evaluate the relationship between the biodegradation and tissue responses by measuring the thickness of residual scaffolds, fibrous capsules and infiltrated tissues through integrated techniques of histology, optical imaging and image analysis. Our results showed that scaffolds with both pore sizes (74.35 ± 10.84 μm and 139.23 ± 44.93 μm, respectively) were well tolerated by host animals and pore size was found to be the rate limiting factor to the biodegradation in the subcutaneous implantation model. In addition, the biodegradation of RSFs was inflammation-mediated to a certain degree and fibroblasts may play a critical role in this process. Overall, such semi-quantitative approach was demonstrated to be a simple and effective method to assess the in vivo degradation rate, and the prepared RSFs were presented to have promising potential in tissue engineering applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 79, 1 October 2017, Pages 161-167
نویسندگان
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