کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5434978 | 1509148 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Using gold nanostars modified pencil graphite electrode as a novel substrate for design a sensitive and selective Dopamine aptasensor
ترجمه فارسی عنوان
با استفاده از الکترود گرافیتی مداد اصلاح شده طلا به عنوان یک بستر جدید برای طراحی حساس و انتخابی آپاتوزین دوپامین
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
بیومتریال
چکیده انگلیسی
For the first time, gold nanostars (GNS) were applied for electrostatic and covalent immobilizing a thiol modified Dopamine aptamer on the pencil graphite electrode and signal amplification. Dopamine aptamer was immobilized on the gold nanostars through electrostatic interaction between negatively charged phosphate groups of aptamer and positively charged gold nanostars and AuS well known covalent interaction. In the presence of Dopamine in the test solution, the charge transfer resistance (RCT) on the electrode surface increased with the increase of the Dopamine concentration due to specific interaction between Dopamine aptamer and Dopamine molecules, which made a barrier for electrons and inhibited the electron-transfer. So, the proposed approach showed a high sensitivity and a wide linearity to Dopamine in the range from 1.0 (± 0.1) to 100.0 (± 0.3) ng Lâ 1 (ppt) with detection and quantification limits of 0.29 (± 0.10) and 0.90 (± 0.08) ng Lâ 1 (ppt), respectively. Finally, the sensor was successfully used for determination of Dopamine in biological (human blood plasma and urine) samples. The results open up the path for manufacturing cost effective aptasensors for other biomedical applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 73, 1 April 2017, Pages 700-708
Journal: Materials Science and Engineering: C - Volume 73, 1 April 2017, Pages 700-708
نویسندگان
Rasoul Pourtaghavi Talemi, Seyed Mehdi Mousavi, Hossein Afruzi,