کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5435127 | 1509145 | 2017 | 9 صفحه PDF | دانلود رایگان |
- Chemical reactions were used for the synthesis of Ag nanosubstrates.
- The SERS and Raman spectra were studied for the KCZ.
- The correlation between the concentrations of KCZ and the SERS signal intensity was investigated.
A new method was developed for the characterization and detection of ketoconazole using surface enhanced Raman spectroscopy (SERS) by designing substrates and performing the bands' assignments. Thus, spherical silver nanoparticles (Ag-NPs) were synthesized by a reduction method and designed as substrates for SERS application. The Ag-NPs were characterized using a scanning electron microscope, Fourier transformed infrared spectroscopy and a high-resolution transmission electron microscope. TEM results indicated that the average size of the Ag-NPs was 15 nm. The UV spectrum showed a maximum absorbance of Ag-NPs at about 400 nm. When Ag-NPs were used as substrates in SERS, the Raman spectra of KCZ showed a significant enhancement of the Raman bands. An important finding is a linear relationship between the logarithmical scale of KCZ concentration and the intensity of the SERS bands, for example at 1050 cmâ 1 of KCZ, which is due to the CN vibration. This was optimized and utilized to develop a calibration curve, which was then used for the detection of the KCZ in real pharmaceutical samples. The method has the advantages of a wide dynamic range with a high coefficient of determination and detection limit calculated based on the signal-to-noise ratio of 3, was 2.6 Ã 10â 10 M and the limit of quantification was 7.8 Ã 10â 10 M. The potential applications that take advantage of the high SERS sensitivity of this method are discussed for practical KCZ analysis where were quantified with this method.
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Journal: Materials Science and Engineering: C - Volume 76, 1 July 2017, Pages 356-364