Adsorption of nisin into layered double hydroxide nanohybrids and in-vitro controlled release

- Adsorption nisin on layered double hydroxide (LDH) was proposed.
- Adsorption performance over the layer charge density, the interlayer anion, the host matrix, and the matrix morphology.
- Release trials of free nisin and nisin loaded LDH were investigated into PBS medium.
- Sustained release profile is achieved from the LDH carrier, offering a way to effectively use of nisin.

Layered double hydroxide (LDH) nanohybrid intercalated biomolecules, including oligonecluotides, genes and peptides/proteins, have attracted particular attention since they exhibit improved safety and effectiveness as successful delivery biosystems. The current study specifically investigated the adsorption of nisin peptide and precisely the control of the release of the payload. Adsorption occurred from peptide solution in contact with zinc-aluminum LDH at room temperature, looking out over the influence of the Zn2 +/Al3 + ratio, the anion exchange capacity, the nature of the intercalated anion, the host matrix, and the host morphology. Higher adsorption was obtained, around 80% of the loaded nisin and successful intercalation was verified by X-ray diffraction. The in-vitro release tests of the nisin from the biohybrid formulation was held over 25 days in PBS medium (0.01 M, pH 7,4) and showed that no burst release phenomenon occurred at the beginning step, in addition, a sustained-time release of nisin was obtained compared with the free nisin. Therefore, these preliminary results are encouraging for the development of bioprotectors based on nisin intercalated LDH and being implemented in the food and medical industries.