کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5456987 | 1515118 | 2017 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
F-actin reorganization upon de- and rehydration in the aeroterrestrial green alga Klebsormidium crenulatum
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کلمات کلیدی
EGTAPAMF-actinMBSCharophytaphalloidin stainingfilamentous actin - actin filamentousRehydration - آب شدن مجددethylene glycol tetraacetic acid - اتیلن گلیکول تتراستیک اسیدdesiccation - خشک کردنrelative air humidity - رطوبت نسبی هواPipes - لوله هایpulse amplitude modulation - مدولاسیون دامنه پالسWater potential - پتانسیل آب
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
دانش مواد (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Filamentous actin (F-actin) is a dynamic network involved in many cellular processes like cell division and cytoplasmic streaming. While many studies have addressed the involvement of F-actin in different cellular processes in cultured cells, little is known on the reactions to environmental stress scenarios, where this system might have essential regulatory functions. We investigated here the de- and rehydration kinetics of breakdown and reassembly of F-actin in the streptophyte green alga Klebsormidium crenulatum. Measurements of the chlorophyll fluorescence (effective quantum yield of photosystem II [ÎF/Fm']) via pulse amplitude modulation were performed as a measure for dehydration induced shut down of physiological activity, which ceased after 141 ± 15 min at â¼84% RH. We hypothesized that there is a link between this physiological parameter and the status of the F-actin system. Indeed, 20 min of dehydration (ÎF/Fm' = 0) leads to a breakdown of the fine cortical F-actin network as visualized by Atto 488 phalloidin staining, and dot-like structures remained. Already 10 min after rehydration a beginning reassembly of F-actin is observed, after 25 min the F-actin network appeared similar to untreated controls, indicating a full recovery. These results demonstrate the fast kinetics of F-actin dis- and reassembly likely contributing to cellular reorganization upon rehydration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Micron - Volume 98, July 2017, Pages 34-38
Journal: Micron - Volume 98, July 2017, Pages 34-38
نویسندگان
Kathrin Blaas, Andreas Holzinger,