کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5475650 1521412 2017 50 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synchronized nutrient stress conditions trigger the diversion of CDP-DG pathway of phospholipids synthesis towards de novo TAG synthesis in oleaginous yeast escalating biodiesel production
موضوعات مرتبط
مهندسی و علوم پایه مهندسی انرژی انرژی (عمومی)
پیش نمایش صفحه اول مقاله
Synchronized nutrient stress conditions trigger the diversion of CDP-DG pathway of phospholipids synthesis towards de novo TAG synthesis in oleaginous yeast escalating biodiesel production
چکیده انگلیسی
In this study oleaginous yeast, Rhodosporidium kratochvilovae HIMPA1 grown in glucose synthetic medium containing different nitrogen (1 g/l and 0.1 g/l) and phosphorus (0.05 g/l and 0.1 g/l) limited conditions. Among various N and P-limited conditions, the highest lipid content (60.34± 0.69%) obtained under synchronized limitation of N and P (0.1 g/l N and 0.05 g/l P). Live fluorescent cell imaging of yeast cells after BODIPY505-515 nm staining endorses the results of triacylglycerol (TAG) accumulation in lipid droplets. The cells grown in synchronized limitation of N and P exhibited boosted cell size (6.76 ± 0.39 μm) and lipid droplet size (5.62 ± 0.28 μm). Under synchronized limitation of N and P, supersized irregular shaped lipid droplets (LD) coalesced to form big lobules in the cellular compartment of oleaginous yeast having 87.14% enhanced TAG accumulation as depicted by TLC. Synchronized nutrient limitation diverts the CDP-DG pathway of phospholipids synthesis towards de novo TAG synthesis. The maximum increment of oleic acid (C18:1) was reported in synchronized limitation of N and P that improve the biodiesel properties like oxidative stability, viscosity, cetane number and cold filter plugging point.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Energy - Volume 139, 15 November 2017, Pages 962-974
نویسندگان
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