کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5478354 | 1521808 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of cell disruption method for lipase extraction from novel thraustochytrids
ترجمه فارسی عنوان
ارزیابی روش خرابی سلولی برای استخراج لیپاز از تریستوشیتریدهای جدید
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی انرژی
انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
چکیده انگلیسی
Marine microorganisms represent a rich source of enzymes with the potential industrial application. In this study, we screened novel thraustochytrids for a range of enzymatic activities. The similarity between isolates based on enzyme activities was investigated using cluster analysis. Thraustochytrids were observed to possess different enzymatic activities, however, we focused on lipase activity. Two strains AMCQ-4b27 (34 IU/g) and AMCQS1-9 (36 IU/g) exhibited highest intracellular lipase activity among the isolates. To extract lipase, four different cell disruption methods such as bead vortexing, grinding, sonication and homogenization, were evaluated for their efficiency on lipase extraction yields and quality. Sonication was found to be the best method for enhancing lipase extraction yields from Thraustochytrium sp. AMCQ-4b27 (0.903 IU/μg) and Schizochytrium AMCQS1-9 (1.44 IU/μg).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Algal Research - Volume 25, July 2017, Pages 62-67
Journal: Algal Research - Volume 25, July 2017, Pages 62-67
نویسندگان
Avinesh R. Byreddy, Nalam Madhusudhana Rao, Colin J. Barrow, Munish Puri,