کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5489130 1524353 2017 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Time lapse microscopy of temperature control during self-assembly of 3D DNA crystals
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم فیزیک ماده چگال
پیش نمایش صفحه اول مقاله
Time lapse microscopy of temperature control during self-assembly of 3D DNA crystals
چکیده انگلیسی
DNA nanostructures are created by exploiting the high fidelity base-pairing interactions of double-stranded branched DNA molecules. These structures present a convenient medium for the self-assembly of macroscopic 3D crystals. In some self-assemblies in this system, crystals can be formed by lowering the temperature, and they can be dissolved by raising it. The ability to monitor the formation and melting of these crystals yields information that can be used to monitor crystal formation and growth. Here, we describe the development of an inexpensive tool that enables direct observation of the crystal growth process as a function of both time and temperature. Using the hanging-drop crystallization of the well-characterized 2-turn DNA tensegrity triangle motif for our model system, its response to temperature has been characterized visually.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Crystal Growth - Volume 476, 15 October 2017, Pages 1-5
نویسندگان
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