کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5498009 1532076 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel Tc-99 m and fluorescence labeled peptide as a multimodal imaging agent for targeting angiogenesis in a murine hindlimb ischemia model
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم تشعشع
پیش نمایش صفحه اول مقاله
A novel Tc-99 m and fluorescence labeled peptide as a multimodal imaging agent for targeting angiogenesis in a murine hindlimb ischemia model
چکیده انگلیسی
The serine-aspartic acid-valine (SDV) peptide binds specifically to integrin αvβ3. We developed a Tc-99 m and TAMRA labeled peptide, Tc-99 m SDV-ECG-K-TAMRA for multimodal imaging of angiogenesis. Tc-99 m SDV-ECG-K-TAMRA was prepared in high yield (>96%) and showed low cytotoxicity. Tc-99 m tetrofosmin images 1 week after operation, revealed significantly decreased perfusion of the ischemic hindlimb, and the perfusion recovered gradually for 4 weeks. In contrast, Tc-99 m SDV-ECG-K-TAMRA uptake was maximal 1 week after the operation (ischemic-to-non-ischemic uptake ratio =5.03±1.01) and decreased gradually. The ischemic-to-non-ischemic ratio of Tc-99 m SDV-ECG-K-TAMRA and Tc-99 m tetrofosmin was strongly negatively correlated (r =−0.94). A postmortem analysis revealed increased angiogenesis markers and uptake of Tc-99 m SDV-ECG-K-TAMRA by ischemic tissue. Our in vivo and in vitro studies revealed substantial uptake of Tc-99 m SDV-ECG-K-TAMRA by ischemic tissue. Tc-99 m SDV-ECG-K-TAMRA could be a good candidate dual-modality imaging agent to assess angiogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Radiation and Isotopes - Volume 121, March 2017, Pages 22-27
نویسندگان
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