کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5508796 1400400 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cullin9 protects mouse eggs from aneuploidy by controlling microtubule dynamics via Survivin
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Cullin9 protects mouse eggs from aneuploidy by controlling microtubule dynamics via Survivin
چکیده انگلیسی


- Cullin9 localizes on the spindle fibers during mouse oocyte meiotic maturation.
- Cullin9 regulates spindle assembly and chromosome alignment in mouse oocyte meiosis.
- Cullin9 is required for kinetochore-microtubule attachment in mouse oocyte meiosis.
- Cullin9 prevents mouse eggs from aneuploidy by regulating microtubule dynamics via Survivin.

The Cullin9 gene encodes a putative E3 ligase that serves a wide variety of biological functions in mitosis, whereas its roles in meiosis have not yet clearly defined. Here, we report that Cullin9 accumulates on the spindle apparatus and colocalizes with the microtubule fibers during mouse oocyte meiotic maturation. Depletion of Cullin9 by morpholino microinjection results in a remarkably higher rate of disorganized spindles and misaligned chromosomes in oocytes, which is coupled with the impaired kinetochore-microtubule attachments. Resultantly, the incidence of aneuploid eggs significantly increases in Cullin9-depleted oocytes. Moreover, we show that Cullin9 controls Survivin's protein level during meiotic maturation, and thus regulates microtubule stability in oocytes. Thus, our study assigns a new meiotic function to Cullin9 and reveals that it prevents mouse eggs from aneuploidy by regulating microtubule dynamics via Survivin.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1863, Issue 12, December 2016, Pages 2934-2941
نویسندگان
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