کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5508830 1400401 2017 42 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Deep sequencing reveals a global reprogramming of lncRNA transcriptome during EMT
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Deep sequencing reveals a global reprogramming of lncRNA transcriptome during EMT
چکیده انگلیسی
Deep-sequencing results showed that the long RNA transcriptome of MCF10A had undergone global changes as early as 8 h after treatment with TGF-β. The expression of 3403 known and novel lncRNAs, and 570 known and novel circRNAs were altered during EMT. To identify the key lncRNA-regulator, we constructed the co-expression network and found all junction nodes in the network are lncRNAs. One junction node, RP6-65G23.5, was further verified as a key regulator of EMT. Intriguingly, we identified 216 clusters containing lncRNAs which were located in “gene desert” regions. The expressions of all lncRNAs in these clusters changed concurrently during EMT, strongly suggesting that these clusters might play important roles in EMT. Our study reveals a global reprogramming of lncRNAs transcriptome during EMT and provides clues for the future study of the molecular mechanism of EMT.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1864, Issue 10, October 2017, Pages 1703-1713
نویسندگان
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