Selenium deficiency-induced thioredoxin suppression and thioredoxin knock down disbalanced insulin responsiveness in chicken cardiomyocytes through PI3K/Akt pathway inhibition

Primary cardiomyocytes culture about 48 h, all cells were divided into two groups, normal group (N) and knock down group (KD), cell samples were collected for detecting the insulin and PI3K pathway-related genes.85