کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5509795 1538635 2017 27 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous determination of plasma total homocysteine and methionine by liquid chromatography-tandem mass spectrometry
ترجمه فارسی عنوان
تعیین همزمان هموسیستئین و متیونین پلاسما با استفاده از اسپکترومتری جرمی کروماتوگرافی-دو طرفه
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
The sulfur-containing amino acid homocysteine is a cardiac risk factor and a biomarker for several inborn errors of metabolism in methionine synthesis. A simple LC-MS/MS method was developed and validated for determination of homocysteine and methionine in human plasma. Rapid separation was achieved using a reverse phase liquid chromatography. Mass spectrometry identification was performed in positive electrospray ionization mode for homocysteine and methionine. Accuracy, precision, linearity, recovery and sample stability were evaluated in the method validation. The test is applied in diagnosis of homocystinuria and monitoring total homocysteine levels. Moreover, simultaneous measurement of methionine helps in the differentiation of homocystinuria and some cobalamin disorders (such as cblC and cblD defects) without additional amino acid testing. Lastly, this assay is sensitive to detect reduced total homocysteine levels that are possibly seen in sulfocysteinuria and molybdenum cofactor deficiencies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 464, January 2017, Pages 93-97
نویسندگان
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