کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5510256 | 1538975 | 2017 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Glutamine protects intestinal calcium absorption against oxidative stress and apoptosis
ترجمه فارسی عنوان
گلوتامین از جذب کلسیم روده در برابر استرس اکسیداتیو و آپوپتوز محافظت می کند
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کلمات کلیدی
Mothers against decapentaplegic homolog 3DABPMCA1bGLNSmad3TGF-β1HspNBTERKGSHCAT3,3′-diaminobenzidine - 3،3'-diaminobenzidineI/R - I / RMAPK - MAPKO2− - O2-ROS - ROS superoxide anion - آنیون سوپر اکسیدischemia/reperfusion - ایسکمی / رپرفیوژنtransforming growth factor beta 1 - تبدیل فاکتور رشد بتا 1nitro blue tetrazolium - تترازولیم نیترو آبیOxidative stress - تنش اکسیداتیوTUNEL - تونلintestinal calcium absorption - جذب کلسیم رودهApoptosis - خزان یاختهایSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازPlasma membrane Ca2+-ATPase - غشای پلاسما Ca2 + -ATPaseMen - مردانmenadione - منادیونHeat shock protein - پروتئین شوک حرارتoptical density - چگالی نوریCatalase - کاتالازCalbindin D28K - کالبندین D28Kextracellular signal-regulated kinases - کیناز های تنظیم شده سیگنال خارج سلولیmitogen-activated protein kinases - کیناز پروتئین فعال MitogenGlutathione - گلوتاتیونglutamine - گلوتامینReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
The aim of this study was to investigate whether glutamine (GLN) could block the inhibition of the intestinal Ca2+ absorption caused by menadione (MEN), and elucidate the underlying mechanisms. To do this, one-month old chicks were divided in four groups: 1) controls, 2) MEN treated, 3) GLN treated and 4) GLN treated before or after MEN treatment. Intestinal Ca2+ absorption as well as protein expression of molecules involved in the transcellular Ca2+ pathway were determined. Glutathione (GSH) and superoxide anion and activity of enzymes of the antioxidant system were evaluated. Apoptosis was measured by the TUNEL technique, the expression of FAS and FASL and the caspase-3 activity. A previous dose of 0.5 g GLN/kg of b.w. was necessary to show its protector effect and a dose of 1 g/kg of b.w. could restore the intestinal Ca2+ absorption after MEN treatment. GLN alone did not modify the protein expression of calbindin D28k and plasma membrane Ca2+-ATPase, but blocked the inhibitory effect of the quinone. GLN avoided changes in the intestinal redox state provoked by MEN such as a decrease in the GSH content, and increases in the superoxide anion and in the SOD and CAT activities. GLN abrogated apoptotic effects caused by MEN in intestinal mucosa, as indicated by the reduction of TUNEL (+) cells and the FAS/FASL/caspase-3 pathway. In conclusion, GLN could be an oral nutritional supplement to normalize the redox state and the proliferation/cell death ratio in the small intestine improving the intestinal Ca2+ absorption altered by oxidative stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 212, October 2017, Pages 64-71
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 212, October 2017, Pages 64-71
نویسندگان
Luciana Moine, Gabriela DÃaz de Barboza, Adriana Pérez, Mercedes Benedetto, Nori Tolosa de Talamoni,