کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5510481 | 1539129 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role of neuropeptide F in regulating filter feeding of Manila clam, Ruditapes philippinarum
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Endogenous signals which may be involved in the regulation of filter feeding in bivalves have never been examined. NPY/NPF homologue has been proved to play an important role in the regulation of food intake in vertebrate and several invertebrates. In this study, a NPF homologue was cloned from visceral ganglia of clam Ruditapes philippinarum. The full-length cDNA sequence was 892 bp in length and encoded a precursor of 82 amino acid residues. We then examined the effects of fasting and refeeding on the filtration rates (FR), plasma glucose concentration (PGC), 5-HT, DA and the expression level of the rp-NPF and insulin transcript. The mRNA expression level of rp-NPF in visceral ganglion was increased during fasting, and rose to highest level on 72 h after starvation and declined immediately after food had been supplied. Hemocoel injection of rp-NPF(5 μg/g)significantly increased FR of clams within 2 h. Compared to the controls, a significant increase in insulin mRNA levels was observed at 8 h after injection. Contents of 5-HT and DA also increased in the 5 μg/grp-NPF administrated clams at 8 and 24 h after injection. These results suggest that, similar to vertebrates, NPF, insulin, 5-HT and DA may play a role in the regulation of feeding in R. philippinarum.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 205, March 2017, Pages 30-38
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 205, March 2017, Pages 30-38
نویسندگان
Xin Wang, Jingjing Miao, Peipei Liu, Luqing Pan,