کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5510643 | 1539271 | 2017 | 49 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bothrops fonsecai snake venom activities and cross-reactivity with commercial bothropic venom
ترجمه فارسی عنوان
فعالیت های سم مار از هر دو جنس در هر دو جنس و واکنش متقابل با سم تجاری هرمی
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کلمات کلیدی
CAVEDLPLA2SAVSE-HPLCSnake Venom Serine proteinasesSVMPAntivenom neutralizationSVSPphospholipase A2 - آنزیم فسفولیپاز A2 Local effects - اثرات محلیSize-exclusion high-performance liquid chromatography - اندازه گیری کروماتوگرافی مایع با کارایی بالاSnake Venom Metalloproteinases - زهر مار، متالوپروتئینازهاExtensor digitorum longus muscle - عضلات بازکننده انگشتان لونگوسEnzymatic activities - فعالیت های آنزیمیNeuromuscular blockade - محرومیت عضلانی عضلانی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
In this work, we examined some biochemical and biological activities of Bothrops fonsecai venom, a pitviper endemic to southeastern Brazil, and assessed their neutralization by commercial bothropic antivenom (CAv). Cross-reactivity of venom with CAv was also assessed by immunoblotting and size-exclusion high performance chromatography (SE-HPLC). Bothrops fonsecai venom had PLA2, proteolytic and esterase activities that were neutralized to varying extents by venom:antivenom ratios of 5:1 and 5:2 (PLA2 and esterase activities) or not significantly by either venom:antivenom ratio (proteolytic activity). The minimum hemorrhagic dose (69.2 μg) was totally neutralized by both ratios. Clotting time in rat citrated plasma was 33 ± 10.5 s (mean ± SD; n = 5) and was completely neutralized by a 5:2 ratio. Edema formation was dose-dependent (1-30 μg/site) and significantly inhibited by both ratios. Venom (10-300 μg/mL) caused neuromuscular blockade in extensor digitorum longus preparations; this blockade was inhibited best by a 5:2 ratio. Venom caused myonecrosis and creatine kinase release in vivo (gastrocnemius muscle) and in vitro (extensor digitorum longus) that was effectively neutralized by both venom:antivenom ratios. Immunoblotting showed that venom components of ~ 25-100 kDa interacted with CAv. SE-HPLC profiles for venom incubated with CAv or specific anti-B. fonsecai antivenom raised in rabbits (SAv) indicated that CAv had a higher binding capacity than SAv, whereas SAv had higher affinity than CAv. These findings indicate that B. fonsecai venom contains various activities that are neutralized to different extents by CAv and suggest that CAv could be used to treat envenoming by B. fonsecai.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 191, January 2017, Pages 86-100
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 191, January 2017, Pages 86-100
نویسندگان
Rita de Cássia O. Collaço, Priscila Randazzo-Moura, Mariana L. Tamascia, Igor Rapp F. da Silva, Thalita Rocha, José C. Cogo, Stephen Hyslop, Charles G. Sanny, Léa Rodrigues-Simioni,