کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5510661 | 1539268 | 2017 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Overexpression of carboxylesterase contributes to the attenuation of cyanotoxin microcystin-LR toxicity
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کلمات کلیدی
Carboxylesterasesbis-p-nitrophenyl phosphatePNPABNPPOATPOATP1B3PNPPVDF3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyl tetrazolium bromideMTT - MTTp-nitrophenyl - p-نیترفنیلp-Nitrophenyl acetate - p-نیترفنیل استاتIrinotecan - ایرینوتکانCES - اینOverexpression - بیش از حد بیانpolyvinylidene difluoride - دی فلوئورید پلی وینیلیدینmicrocystin-LR - میکروسیستین LRProtein phosphatase - پروتئین فسفاتازOrganic anion transporting polypeptide - پلیپپتید حمل آنیون ارگانیکcarboxylesterase - کربوکسی استراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Microcystin-LR is a hepatotoxin produced by several cyanobacteria. Its toxicity is mainly due to a inhibition of protein phosphatase, PP1 and PP2A. Previously, we used a cell line stably expressing uptake transporter for microcystin-LR, OATP1B3 (HEK293-OATP1B3 cells). In this study, to determine whether overexpression of carboxylesterase (CES), which degrades ester-group and amide-group, attenuates the cytotoxicity of microcystin-LR, we generated the HEK293-OATP1B3/CES2 double-transfected cells. HEK293-OATP1B3/CES2 cells showed high hydrolysis activity of p-nitrophenyl acetate (PNPA), which is an authentic substrate for esterase. CES activity in HEK293-OATP1B3/CES2 cells was approximately 3-fold higher than that in the HEK293-OATP1B3 cells. HEK293-OATP1B3/CES2 cells (IC50: 25.4 ± 7.7 nM) showed approximately 2.1-fold resistance to microcystin-LR than HEK293-OATP1B3 cells (IC50: 12.0 ± 1.5 nM). Moreover, the CES inhibition assay and microcystin-agarose pull down assay showed the possibility of the interaction between CES2 and microcystin-LR. Our results indicated that the overexpression of CES2 attenuates the cytotoxicity of microcystin-LR via interaction with microcystin-LR.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 194, April 2017, Pages 22-27
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 194, April 2017, Pages 22-27
نویسندگان
Shota Takumi, Tai Shimono, Satoshi Ikema, Yuki Hotta, Petros K. Chigwechokha, Kazuhiro Shiozaki, Yasumasa Sugiyama, Mitsuru Hashimoto, Tatsuhiko Furukawa, Masaharu Komatsu,