کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5511446 1539858 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterising live cell behaviour: Traditional label-free and quantitative phase imaging approaches
ترجمه فارسی عنوان
ویژگی رفتار سلول زنده: رویکردهای تصویربرداری فازی سنتی و بدون برچسب
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


- Dyes, labels and phototoxic routines used to generate image contrast can modify native cell behaviour.
- Label-free imaging uses optical setups that create contrast by exploiting changes in phase delay (thickness/biomolecule composition) of cells.
- In contrast to traditional label-free techniques (PC or DIC), quantitative phase imaging quantifies the phase delay and is high-contrast.
- High-contrast images allow segmentation/tracking algorithms to work with fewer constraints, facilitating robust extraction of cell metrics.
- Label-free dynamic phenotypes contain individual cell metrics, enhancing detection of behavioural changes compared to population averages.

Label-free imaging uses inherent contrast mechanisms within cells to create image contrast without introducing dyes/labels, which may confound results. Quantitative phase imaging is label-free and offers higher content and contrast compared to traditional techniques. High-contrast images facilitate generation of individual cell metrics via more robust segmentation and tracking, enabling formation of a label-free dynamic phenotype describing cell-to-cell heterogeneity and temporal changes. Compared to population-level averages, individual cell-level dynamic phenotypes have greater power to differentiate between cellular responses to treatments, which has clinical relevance e.g. in the treatment of cancer. Furthermore, as the data is obtained label-free, the same cells can be used for further assays or expansion, of potential benefit for the fields of regenerative and personalised medicine.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 84, March 2017, Pages 89-95
نویسندگان
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