کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5511965 | 1540217 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Engineering a xylanase from Streptomyce rochei L10904 by mutation to improve its catalytic characteristics
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Engineering a xylanase from Streptomyce rochei L10904 by mutation to improve its catalytic characteristics Engineering a xylanase from Streptomyce rochei L10904 by mutation to improve its catalytic characteristics](/preview/png/5511965.png)
چکیده انگلیسی
Protein engineering was performed by N-terminal region replacement and site-directed mutagenesis in the cord of a xylanase (Srxyn) from Streptomyce rochei L10904 to improve its catalytic characteristics. Three mutants SrxynF, SrxynM and SrxynFM displayed 2.1-fold, 3.2-fold and 5.3-fold higher specific activities than that of Srxyn, respectively. Moreover, all of the mutants showed greater substrate affinity and kcat/Km than the native Srxyn. In addition, the enzymes showed improved hydrolysis characteristics, of which the most noteworthy is the enhanced ability of producing xylobiose (X2) and xylotriose (X3) from polymeric substrates. The engineered xylanases have greater potential for applications in oligosaccharide preparation industry.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 101, August 2017, Pages 366-372
Journal: International Journal of Biological Macromolecules - Volume 101, August 2017, Pages 366-372
نویسندگان
Qin Li, Baoguo Sun, Huiyong Jia, Jie Hou, Ran Yang, Ke Xiong, Youqiang Xu, Xiuting Li,