کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5512139 1540226 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fabrication of FGF-2 immobilized electrospun gelatin nanofibers for tissue engineering
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Fabrication of FGF-2 immobilized electrospun gelatin nanofibers for tissue engineering
چکیده انگلیسی

Conjugated gelatin nanofibers were fabricated by electrospinning, followed by a simple glutaraldehyde cross-linking procedure and avidin conjugation. Then, biotinylated growth factors were immobilized onto the surface of the fibers through avidin-biotin covalent binding. The immobilization of growth factors was confirmed through immunostaining using fluorescence microscopy and microplate spectrophotometry. Adipose derived stem cells (ASCs) were cultured to examine the effect of immobilized growth factors on cell proliferation using the cell counting Kit-8 (CCK-8) assay. Gelatin nanofibers with no growth factors attached and growth factors in suspension within media were used as controls. Growth factors were successfully immobilized onto the surface, in amounts corresponding to the concentrations applied, and increased cell proliferation to a higher extend than growth factors in suspension. Our results suggest that this controllable scaffolding strategy provides an effective system for growth factors delivery in tissues, suitable for engineering applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 93, Part B, December 2016, Pages 1559-1566
نویسندگان
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