کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5512382 1540223 2017 40 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization and immunomodulatory activities of polysaccharides extracted from green alga Chlorella ellipsoidea
ترجمه فارسی عنوان
خصوصیات و فعالیت های ایمونوژنتیک پلی ساکارید استخراج شده از جلبک سبز کلرلا اپی فایده
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Polysaccharides of green alga Chlorella ellipsoidea were extracted using hot water and fractionated using an anion-exchange chromatography to investigate their molecular characteristics and immunomodulatory activities. The crude polysaccharide and two fractions (F1 and F2) consisted mainly of carbohydrate (68.1-89.7%) and protein (2.0-11.8%) with small amounts of sulfate (1.9-6.1%) and uronic acid (0.5-6.1%). Glucose (58.8-97.6%) was the major monosaccharide of these polysaccharides, with different levels of rhamnose (0.2-11.6%), mannose (0.4-2.6%), and galactose (1.8-27.0%). The average molecular weights (Mw) of the crude, F1, and F2 were 175.8 × 103, 126.9 × 103, and 237.0 × 103 g/mol, respectively. The crude, F1, and F2 stimulated murine macrophage, RAW264.7 cells, to produce considerable amounts of nitric oxide (NO) and various cytokines via up-regulation of their mRNA expression by the activation of nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. The fraction F2 with higher Mw and protein content showed stronger immunomodulatory activity. The main backbone of the fraction F2 was mainly connected via (1 → 4)-linked glucose and (1 → 6)-linked galactose with branches at C-3 and C-4 positions in (1 → 3,4)-linked glucose and (1 → 4,6)-linked galactose residues, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 95, February 2017, Pages 106-114
نویسندگان
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