کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5513372 1541201 2017 27 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Use of two-photon microscopy to study Leishmania major infection of the skin
ترجمه فارسی عنوان
استفاده از میکروسکوپ دو فوتون برای بررسی عفونت بزرگ لیشمانیا پوست
کلمات کلیدی
لیشمانیا، تصویربرداری پوست گوش، شن و ماسه پرواز، نوتروفیل، موش های خبرنگار،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Intra-vital two-photon microscopy (2P-IVM) allows for in-situ investigation of tissue organization, cell behavior and the dynamic interactions between different cell types in their natural environment. This methodology has also expanded our understanding of the immune response against pathogens. Leishmania are protozoan intracellular parasites that have adapted to successfully establish infection within the context of an inflammatory response in the skin following transmission by the bite of an infected sand fly. The generation of fluorescent transgenic parasites coupled with the increased availability of different types of fluorescent transgenic reporter mice has facilitated the study of the host-parasite interaction in the skin, significantly impacting our understanding of cutaneous leishmaniasis. In this review we will discuss 2P-IVM in the context of Leishmania infection of the mouse ear skin and describe a simple and minimally invasive procedure that allows long-term imaging of this host-pathogen interaction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 127, 15 August 2017, Pages 45-52
نویسندگان
, , , ,