کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5514228 1541593 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous monitoring of intra- and extracellular nitric oxide in living cells by means of dual-color fluorescence imaging
ترجمه فارسی عنوان
نظارت همزمان اکسید نیتریک داخل و خارج سلولی در سلول های زنده با استفاده از تصویربرداری فلورسانس دو رنگ
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


- A method based on dual-color fluorescence imaging was developed for simultaneous monitoring of intra- and extracellular NO.
- The two probes with the same trapper for NO could reveal the amounts of NO within and outside the cell more accurately.
- The two probes display distinct membrane permeability, and show different colors of fluorescence after reaction with NO.
- The proposed method was validated by simultaneously detecting intra- and extracellular NO in living cells.

A dual-color fluorescence imaging method for simultaneous monitoring of intra- and extracellular nitric oxide (NO) was developed. Assisted by confocal laser scanning microscope, the intra- and extracellular NO can be successfully visualized by using two selected probes, 4,4-difluoro-8-(3,4-diaminophenyl)-3,5-bis(4-methoxyphenyl)-4-bora-3a,4a-diaza-s-indacene (p-MOPB) and disodium 2,6-disulfonate-1,3-dimethyl-5-hexadecyl-8-(3,4-diaminophenyl)-4,4'-difluoro-4-bora-3a,4a-diaza-s-indacene (DSDMHDAB), which display distinct membrane permeability and show different colors of fluorescence after reaction with NO. Results indicated that intra- and extracellular NO could be fluorometrically detected without mutual interference. The applicability of the proposed method was validated by dual-color imaging of NO on both sides of the plasma membrane in RAW 264.7 murine macrophages and human vascular endothelial (ECV-304) cells. This multi-labeling approach using multi-laser excitation and multi-color fluorescence detection holds great promise for simultaneous analysis of NO as well as other gasotransmitters in living cells with subcellular resolution.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nitric Oxide - Volume 67, 1 July 2017, Pages 30-38
نویسندگان
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