Cu from dissolution of CuO nanoparticles signals changes in root morphology

• CuO NPs and Cu ions inhibit root elongation in wheat seedlings.
• CuO NPs associate with the root surface and root hairs.
• CuO NPs and Cu ions alter root epidermal cell lineage and differentiation to promote root hair formation.
• Neither Cu treatments modify epidermal cell death or nitric oxide accumulation.

Utilization of CuO nanoparticles (NPs) in agriculture, as fertilizers or pesticides, requires understanding of their impact on plant metabolism. Inhibition of root elongation by CuO NPs (>10 mg Cu/kg) occurred in wheat grown in sand. Morphological changes included root hair proliferation and shortening of the zones of division and elongation. The epidermal cells in the compressed root tip were abnormal in shape and file patterning but staining with SYTOX Blue did not reveal a general increase in epidermal cell death. Inhibition of root elongation and proliferation of root hair formation occurred also in response to exogenous indole acetic acid (IAA) supplied through tryptophan metabolism by the root-colonizing bacterium, Pseudomonas chlororaphis O6. Altered root morphology caused by the CuO NPs was likely due to release of Cu from dissolution at the root surface because similar changes occurred with Cu ions (≥6 mg/kg). Use of a fluorescent probe showed the accumulation of nitric oxide (NO), required for root hair formation, was not changed by the NPs. These findings suggested that dissolution of the NPs in the rhizosphere resulted levels of Cu that modified IAA distribution to causing root shortening but permitted NO cell signaling to promote root hair proliferation.

CuO NPs form aggregates on the plant root surfaces. NP dissolution, enhanced by factors in the rhizosphere such as organic acids, release Cu, causing root shortening and proliferation of the root hairs close to the root tip. These responses may be due to changes in IAA redistribution from the base of the stele that result in enhanced differentiation of root epidermal cells into elongated root hairs.Figure optionsDownload high-quality image (133 K)Download as PowerPoint slide