کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5515514 1541909 2017 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparative analysis of mutant plants impaired in the main regulatory mechanisms of photosynthetic light reactions - From biophysical measurements to molecular mechanisms
ترجمه فارسی عنوان
تجزیه و تحلیل مقایسه ای از گیاهان جهش یافته در مکانیسم های اصلی تنظیم کننده واکنش های نورسنجی فتوسنتز - از اندازه گیری های بیوفیزیکی به مکانیزم های مولکولی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
چکیده انگلیسی


- Comparative fluorescence and P700 analysis of photosynthesis regulation mutants clarifies the origin of Dual-PAM signals.
- A clear molecular mechanism-specific behavior of distinct original fluorescence signals is demonstrated.
- The molecular mechanism-specific information easily disappears upon mathematical treatment of original fluorescence data.

Chlorophyll (chl) fluorescence emission by photosystem II (PSII) and light absorption by P700 reaction center chl a of photosystem I (PSI) provide easy means to probe the function of the photosynthetic machinery. The exact relationship between the measured optical variables and the molecular processes have, however, remained elusive. Today, the availability of mutants with distinct molecular characterization of photosynthesis regulatory processes should make it possible to gain further insights into this relationship, yet a systematic comparative analysis of such regulatory mutants has been missing. Here we have systematically compared the behavior of Dual-PAM fluorescence and P700 variables from well-characterized photosynthesis regulation mutants. The analysis revealed a very convincing relationship between the given molecular deficiency in the photosynthetic apparatus and the original fluorescence and P700 signals obtained by using varying intensities of actinic light and by applying a saturating pulse. Importantly, the specific information on the underlying molecular mechanism, present in these authentic signals of a given photosynthesis mutant, was largely nullified when using the commonly accepted parameters that are based on further treatment of the original signals. Understanding the unique relationship between the investigated molecular process of photosynthesis and the measured variable is an absolute prerequisite for comprehensive interpretation of fluorescence and P700 measurements. The data presented here elucidates the relationships between the main regulatory mechanisms controlling the photosynthetic light reactions and the variables obtained by fluorescence and P700 measurements. It is discussed how the full potential of optical photosynthesis measurements can be utilized in investigation of a given molecular mechanism.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 112, March 2017, Pages 290-301
نویسندگان
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