Overexpression and characterization of a novel endo-β-1,3(4)-glucanase from thermophilic fungus Humicola insolens Y1

- HiCel16A is the first β-1,3(4)-glucanase characterized from Humicola insolens.
- Recombinant HiCel16A was successfully overexpressed in Pichia pastoris and showed optimal activity at pH 5.5 and 55 °C.
- HiCel16A exhibited outstanding stability over pH 5.0-9.0 and at temperatures up to 50 °C, remaining >80% of the peak activity.

A novel endo-β-1,3(4)-glucanase gene, cel16A, was cloned from the fungus Humicola insolens Y1. The 988-bp full-length gene encoded a 286-residue polypeptide consisting of a putative signal peptide of 20 residues and a catalytic domain belonging to glycosyl hydrolase family 16. It was successfully overexpressed in Pichia pastoris GS115. The purified recombinant Cel16A exhibited highest specific activity toward barley β-glucan, followed by lichenan and laminarin, but not toward CMC-Na, birchwood xylan, Avicel and filter paper, indicating that Cel16A is an endo-β-1,3(4)-glucanases. Recombinant Cel16A had a pH optimum at 5.5 and a temperature optimum at 55 °C with a specific activity of 693 U/mg toward barley β-glucan. It exhibited good stability over pH 5.0-9.0 and at temperatures up to 50 °C, retaining over 80% maximum activity. The Km and Vmax values of Cel16A for barley β-glucan were 0.91 mg ml−1 and 1530 μmol min−1·mg−1, respectively. All these favorable enzymatic properties of Cel16A make it a good candidate for applications in various industries.