کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5520567 1544903 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Partitioning and extraction protease from Aspergillus tamarii URM4634 using PEG-citrate aqueous two-phase systems
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Partitioning and extraction protease from Aspergillus tamarii URM4634 using PEG-citrate aqueous two-phase systems
چکیده انگلیسی

PEG-citrate Aqueous Two-Phase Systems (ATPS) were used to recover and partially purify protease from Aspergillus tamarii URM4634 produced by Solid State Fermentation. Experiments were performed according to a 24-full factorial design using PEG molar mass (MPEG), PEG concentration (CPEG), citrate concentration (CCIT) and pH as independent variables; and purification factor (PF), partition coefficient (K) and activity yield (Y) as responses. Protease showed high activity in the PEG-rich phase, also MPEG and CCIT were shown to exert positive effects on all responses. The highest purification factor (3.95) was obtained using MPEG=8000 g/mol, 24% (w/w) CPEG, 20% (w/w) CCIT at pH 8.0. Consequently, the selected ATPS proved to be efficient and can be used as a first step for pre-purification of protease from solid state fermented of A. tamarii URM4634.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biocatalysis and Agricultural Biotechnology - Volume 9, January 2017, Pages 168-173
نویسندگان
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