Rapid typing of Mannheimia haemolytica major genotypes 1 and 2 using MALDI-TOF mass spectrometry

• Genotype 1 and 2 M. haemolytica have distinct mass spectrum profiles.
• A MALDI-TOF MS assay was developed that identifies M. haemolytica genotypes 1 and 2.
• The assay can be easily adapted into diagnostic labs using conventional MALDI-TOF MS.

Genotype 2 M. haemolytica predominantly associate over genotype 1 with the lungs of cattle with respiratory disease and ICEs containing antimicrobial resistance genes. Distinct protein masses were detected by MALDI-TOF MS between genotype 1 and 2 strains. MALDI-TOF MS could rapidly differentiate genotype 2 strains in veterinary diagnostic laboratories.