NoteLive-cell imaging of Salmonella Typhimurium interaction with zebrafish larvae after injection and immersion delivery methods

- A novel protocol for infecting zebrafish larvae with S. Typhimurium by static immersion is described.
- Our infection protocol in zebrafish allows live-cell imaging of Salmonella colonization and zebrafish immune responses.
- Zebrafish larvae is a valid model for rapid, non-invasive real-time analysis of Salmonella infections in a vertebrate host.

The zebrafish model has been used to determine the role of vertebrate innate immunity during bacterial infections. Here, we compare the in vivo immune response induced by GFP-tagged Salmonella Typhimurium inoculated by immersion and microinjection in transgenic zebrafish larvae. Our novel infection protocols in zebrafish allow live-cell imaging of Salmonella colonization.

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